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rat s9-mix  (TRINOVA Biochem)

 
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    TRINOVA Biochem rat s9-mix
    Rat S9 Mix, supplied by TRINOVA Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat s9-mix/product/TRINOVA Biochem
    Average 90 stars, based on 1 article reviews
    rat s9-mix - by Bioz Stars, 2026-02
    90/100 stars

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    Image Search Results


    In vitro bacterial reverse mutation test of the PMR-W-treated TA1537 strain in the absence and presence of metabolic activation (S9 mix). The mean number of revertant colonies in the absence or presence of uninduced is 5% Aroclor 1254-induced rat or 5% human liver S9 mix. Regardless of whether the S9 metabolic activation system was used, no turbidity, precipitation, or antibacterial toxicity, such as the formation of a background lawn, micro-colonies, or a decrease in the number of reverted colonies by more than 50%, was observed in all strains. The data represent the averages from triplicate plates per dose.

    Journal: Scientific Reports

    Article Title: Differential genotoxicity of Polygoni Multiflori in rat and human: insights from Ames test and S9 metabolic activation system

    doi: 10.1038/s41598-024-72283-5

    Figure Lengend Snippet: In vitro bacterial reverse mutation test of the PMR-W-treated TA1537 strain in the absence and presence of metabolic activation (S9 mix). The mean number of revertant colonies in the absence or presence of uninduced is 5% Aroclor 1254-induced rat or 5% human liver S9 mix. Regardless of whether the S9 metabolic activation system was used, no turbidity, precipitation, or antibacterial toxicity, such as the formation of a background lawn, micro-colonies, or a decrease in the number of reverted colonies by more than 50%, was observed in all strains. The data represent the averages from triplicate plates per dose.

    Article Snippet: Briefly, S. typhimurium strains TA98, TA100, TA1535, and TA1537 and E. coli WP2 uvrA (Molecular Toxicology Inc., USA) were used as the test strains in the presence or absence of 5% v/v Aroclor 1254-induced rat liver S9 metabolic system (S9 mix).

    Techniques: In Vitro, Mutagenesis, Activation Assay

    Network analysis using STRING. Identified CYP- and UGT-metabolism-related enzymes from ( A ) human S9 (40 CYPs and UGT in total) and ( B ) Aroclor 1254-induced rat S9 (55 CYPs and UGT in total) were analyzed for each protein–protein interaction network. Metabolism-related enzymes, specifically CYP and UGT, were identified from ( A ) human S9 (comprising a total of 40 CYPs and UGTs) and ( B ) Aroclor 1254-induced rat S9 (with a total of 55 CYPs and UGTs). Each enzyme was analyzed within the protein–protein interaction network.

    Journal: Scientific Reports

    Article Title: Differential genotoxicity of Polygoni Multiflori in rat and human: insights from Ames test and S9 metabolic activation system

    doi: 10.1038/s41598-024-72283-5

    Figure Lengend Snippet: Network analysis using STRING. Identified CYP- and UGT-metabolism-related enzymes from ( A ) human S9 (40 CYPs and UGT in total) and ( B ) Aroclor 1254-induced rat S9 (55 CYPs and UGT in total) were analyzed for each protein–protein interaction network. Metabolism-related enzymes, specifically CYP and UGT, were identified from ( A ) human S9 (comprising a total of 40 CYPs and UGTs) and ( B ) Aroclor 1254-induced rat S9 (with a total of 55 CYPs and UGTs). Each enzyme was analyzed within the protein–protein interaction network.

    Article Snippet: Briefly, S. typhimurium strains TA98, TA100, TA1535, and TA1537 and E. coli WP2 uvrA (Molecular Toxicology Inc., USA) were used as the test strains in the presence or absence of 5% v/v Aroclor 1254-induced rat liver S9 metabolic system (S9 mix).

    Techniques:

    Comparison of metabolic activation systems in the PMR-W-treated TA1537 strain in the Ames test. The mean of the number of revertant colonies in the absence and presence of the 5% Aroclor1254-induced rat S9, 5% human liver S9, 5% and 10% CYP1A1 bactosome (instead of liver s9); combination of 5% human liver S9 and 5% CYP1A1 bactosome; and 10%, 20%, and 40% in vivo BaP-treated rat liver S9 are shown in ( A ). The BaP responses are shown in ( B ). 2AA 0.5 μg/plate (S9 positive control), 9AA 50 μg/plate (positive control), and BaP (Aroclor 1254-induced rat S9 positive control) were treated with positive control.

    Journal: Scientific Reports

    Article Title: Differential genotoxicity of Polygoni Multiflori in rat and human: insights from Ames test and S9 metabolic activation system

    doi: 10.1038/s41598-024-72283-5

    Figure Lengend Snippet: Comparison of metabolic activation systems in the PMR-W-treated TA1537 strain in the Ames test. The mean of the number of revertant colonies in the absence and presence of the 5% Aroclor1254-induced rat S9, 5% human liver S9, 5% and 10% CYP1A1 bactosome (instead of liver s9); combination of 5% human liver S9 and 5% CYP1A1 bactosome; and 10%, 20%, and 40% in vivo BaP-treated rat liver S9 are shown in ( A ). The BaP responses are shown in ( B ). 2AA 0.5 μg/plate (S9 positive control), 9AA 50 μg/plate (positive control), and BaP (Aroclor 1254-induced rat S9 positive control) were treated with positive control.

    Article Snippet: Briefly, S. typhimurium strains TA98, TA100, TA1535, and TA1537 and E. coli WP2 uvrA (Molecular Toxicology Inc., USA) were used as the test strains in the presence or absence of 5% v/v Aroclor 1254-induced rat liver S9 metabolic system (S9 mix).

    Techniques: Comparison, Activation Assay, Ames Test, In Vivo, Positive Control

    CYP1A1 expression levels from BaP-orally treated rat liver S9 faction. CYP1A1 gene expression level in BaP-treated or control corn oil-treated SD rats compared with the Aroclor 1254-induced Rat S9, HEK 293T cell lysate, Human S9, and ezCYP1A1 bactosome ( A ), and CYP1A1/GAPDH ratio ( B ). GAPDH was normalized as an internal control.

    Journal: Scientific Reports

    Article Title: Differential genotoxicity of Polygoni Multiflori in rat and human: insights from Ames test and S9 metabolic activation system

    doi: 10.1038/s41598-024-72283-5

    Figure Lengend Snippet: CYP1A1 expression levels from BaP-orally treated rat liver S9 faction. CYP1A1 gene expression level in BaP-treated or control corn oil-treated SD rats compared with the Aroclor 1254-induced Rat S9, HEK 293T cell lysate, Human S9, and ezCYP1A1 bactosome ( A ), and CYP1A1/GAPDH ratio ( B ). GAPDH was normalized as an internal control.

    Article Snippet: Briefly, S. typhimurium strains TA98, TA100, TA1535, and TA1537 and E. coli WP2 uvrA (Molecular Toxicology Inc., USA) were used as the test strains in the presence or absence of 5% v/v Aroclor 1254-induced rat liver S9 metabolic system (S9 mix).

    Techniques: Expressing, Control